BD HorizonTM RB780 Mouse Anti-Human FoxA2

Description

Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human FoxA2 antibody Cat. No. 571077/571080; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human FoxA2 antibody Cat. No. 571077/571080; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Flow cytometric analysis of FoxA2 expression in Hep G2 cells. Cells from the Human Hep G2 HEPG2 Hepatocellular carcinoma, ATCC HB-8065 cell line were fixed 10 min, 37oC with pre-warmed BD Cytofix Buffer Cat. No. 554655 and then permeabilized on ice, 30 min with BD Phosflow Perm Buffer III Cat. No. 558050 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and stained with either BD Horizon RB780 Mouse IgG1, kappa Isotype Control Cat. No. 568532; dashed line histogram or BD Horizon RB780 Mouse Anti-Human FoxA2 antibody Cat. No. 571077/571080; solid line histogram . The fluorescence histogram showing FoxA2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.