BD HorizonTM RB744 Rat Anti-Mouse Siglec-H

Description

Multiparameter flow cytometric analysis of Siglec-H expression on Mouse leukocytes. SJL Mouse splenocytes Panel A; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 and BALB/c thymic leukocytes Panel B were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Top Plots or BD Horizon RB744 Rat Anti-Mouse Siglec-H antibody Cat. No. 570576/570664; Bottom Plots at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multiparameter flow cytometric analysis of Siglec-H expression on Mouse leukocytes. SJL Mouse splenocytes Panel A; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 and BALB/c thymic leukocytes Panel B were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Top Plots or BD Horizon RB744 Rat Anti-Mouse Siglec-H antibody Cat. No. 570576/570664; Bottom Plots at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multiparameter flow cytometric analysis of Siglec-H expression on Mouse leukocytes. SJL Mouse splenocytes Panel A; preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody/Mouse BD Fc Block, Cat. No. 553141/553142 and BALB/c thymic leukocytes Panel B were stained with PE Rat Anti-Mouse CD45R/B220 Cat. No. 553090 and with either BD Horizon RB744 Rat IgG2b, kappa Isotype Control Cat. No. 570521; Top Plots or BD Horizon RB744 Rat Anti-Mouse Siglec-H antibody Cat. No. 570576/570664; Bottom Plots at 0.25 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. Bivariate pseudocolor density plots showing the correlated expression of Siglec-H or Ig Isotype control staining versus CD45R/B220 were derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leukocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.