BD HorizonTM RB744 Biosimilar Anti-Human TNF

Description

Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Show More Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software. Multiparameter flow cytometric analysis of TNF Adalimumab Biosimilar expression by stimulated Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were stimulated for 5 hours with Phorbol 12-Myristate 13-Acetate Sigma P-8139; 50 ng/ml final concentration and Ionomycin Sigma I-0634; 1 mug/ml final concentration in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were harvested, washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization using BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were then stained with either BD OptiBuild RB744 IgG1, kappa N297A Human Isotype Control Cat. No. 756837; Left Plot or BD Horizon RB744 Biosimilar Anti-Human TNF antibody Cat. No. 571634/571713; Right Plot in BD Perm/Wash Buffer. The bivariate pseudocolor density plot showing the correlated expression of TNF or Ig Isotype control staining versus forward light scatter FSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 Cell Analyzer System and FlowJo Software.