BD HorizonTM RB705 Rat Anti-Mouse CD16/CD32

Description

Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD16/32 expression on viable Mouse splenic lymphocytes. BALB/c Mouse splenic leukocytes were stained with APC Armenian Hamster Anti-Mouse CD3e antibody Cat. No. 553066 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Mouse CD16/CD32 antibody Cat. No. 570588/570676; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD16/CD32 or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Flow cytometry and data analysis were performed using a BD FACSymphony A5 SE Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.