BD HorizonTM RB705 Rat Anti-Human IL-2

Description

Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Show More Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Show More Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software. Two color flow cytometric analysis of IL-2 expression by activated Human peripheral blood lymphocytes. Peripheral blood mononuclear cells were stimulated 5 h with Phorbol 12-Myristate 13-Acetate PMA, Sigma P-8139; 50 ng/ml and Calcium Ionophore A23187 Sigma C-9275; 1 mu g/ml , in the presence of BD GolgiStop Protein Transport Inhibitor Monensin Cat. No. 554724 . The cells were washed with BD Pharmingen Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 followed by washing and permeabilization with BD Perm/Wash Buffer Cat. No. 554723 . The permeabilized cells were stained in BD Perm/Wash Buffer with BD Horizon BV421 Mouse Anti-Human CD3 antibody Cat. No. 562426 and with either BD Horizon RB705 Rat IgG2a, kappa Isotype Control Cat. No. 570262; Left Plot or BD Horizon RB705 Rat Anti-Human IL-2 antibody Cat. No. 570624/570711; Right Plot using BD Biosciences Intracellular Cytokine Staining Protocol. The pseudocolor density plot showing the correlated expression of IL-2 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Flow Cytometer System and FlowJo Software.