BD HorizonTM RB670 Mouse Anti-Human CD45RO

Description

Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with Alexa Fluor 700 Mouse Anti-Human CD45RA antibody Cat. No. 560673 and with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RO antibody Cat. No. 571921/571922; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with Alexa Fluor 700 Mouse Anti-Human CD45RA antibody Cat. No. 560673 and with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RO antibody Cat. No. 571921/571922; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Show More Multiparameter flow cytometric analysis of CD45RO expression on Human peripheral blood leukocyte populations. Human whole blood was stained with Alexa Fluor 700 Mouse Anti-Human CD45RA antibody Cat. No. 560673 and with either BD Horizon RB670 Mouse IgG2a, kappa Isotype Control Cat. No. 571958; Left Plots or BD Horizon RB670 Mouse Anti-Human CD45RO antibody Cat. No. 571921/571922; Right Plots . Erythrocytes were lysed with BD Pharm Lyse Lysing Buffer Cat. No. 555899 . Samples were acquired using the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.10 Software. Top Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus side light-scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocytes. Bottom Plots: The bivariate pseudocolor density plot showing CD45RO expression or Ig Isotype control staining versus CD45RA was derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.