BD HorizonTM RB613 Mouse Anti-Human FoxP3

Description

Multicolor flow cytometric analysis of FoxP3 expression in Human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424; Top Plots and Alexa Fluor 700 Anti-Human CD25 Cat. No. 561398; Bottom Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human FoxP3 antibody Cat. No. 571236/571300; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 or Ig Isotype control staining versus either CD4 or CD25 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Multicolor flow cytometric analysis of FoxP3 expression in Human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424; Top Plots and Alexa Fluor 700 Anti-Human CD25 Cat. No. 561398; Bottom Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human FoxP3 antibody Cat. No. 571236/571300; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 or Ig Isotype control staining versus either CD4 or CD25 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Show More Multicolor flow cytometric analysis of FoxP3 expression in Human lymphocytes. Human peripheral blood mononuclear cells were stained with BD Horizon BV421 Mouse Anti-Human CD4 Cat. No. 562424; Top Plots and Alexa Fluor 700 Anti-Human CD25 Cat. No. 561398; Bottom Plots antibodies. The cells were washed, fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574 . The cells were then stained with either BD Horizon RB613 Mouse IgG1, kappa Isotype Control Cat. No. 571106; Left Plots or BD Horizon RB613 Mouse Anti-Human FoxP3 antibody Cat. No. 571236/571300; Right Plots . Bivariate pseudocolor density plots showing the correlated expression of FoxP3 or Ig Isotype control staining versus either CD4 or CD25 were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software.