BD HorizonTM RB545 Mouse Anti-Human IgG

Description

Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Show More Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software. Multicolor flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody Cat. No. 555415/561742 and with either BD Horizon RB545 Mouse IgG1, kappa Isotype Control Cat. No. 569284; Left Plot or BD Horizon RB545 Mouse Anti-Human IgG antibody Cat. No. 569735/569763; Right Plot . DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of IgG1 or Ig Isotype control staining versus CD19 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative lymphocytes. Samples were acquired on the BD FACSymphony A5 SE Cell Analyzer and spectrally unmixed using FlowJo v10.8 Software.