BD HorizonTM R718 Rat Anti-Mouse IL-17A

Description

Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of IL-17A expression in stimulated Mouse EL-4 cells. Cells from the mouse EL4 T lymphoma, ATCC TIB-39 cell line were stimulated 5 hr with Phorbol 12-Myristate 13-Acetate PMA; 50 ng/ml final concentration; Sigma, Cat. No. P-8139 and Ionomycin 1000 ng/ml final concentration; Sigma, Cat. No. I-0634 in the presence of BD GolgiStop Protein Transport Inhibitor containing Monensin Cat. No. 554724 . The cells were washed with Stain Buffer FBS Cat. No. 554656 and fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The fixed cells were washed and then permeabilized and stained in BD Perm/Wash Buffer Cat. No. 554723 with BD Horizon R718 Rat IgG1 kappa Isotype Control Cat. No. 566948; Left Plot or BD Horizon R718 Rat Anti-Mouse IL-17A antibody Cat. No. 567098; Right Plot at 0.25 mug/test using BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-17A or Ig Isotype control staining versus side-light scatter SSC-A signals was derived from gated events with the forward and side light-scatter characteristics of intact stimulated leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.