BD HorizonTM R718 Rat Anti-Mouse CD86 (B7-2)

Description

Flow cytometric analysis of CD86 B7-2 expression on viable activated Mouse splenic leukocytes. BALB/c splenic leukocytes were activated with lipopolysaccharide LPS; 72 hrs . The activated cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 570981/571052; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 B7-2 expression on viable activated Mouse splenic leukocytes. BALB/c splenic leukocytes were activated with lipopolysaccharide LPS; 72 hrs . The activated cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 570981/571052; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD86 B7-2 expression on viable activated Mouse splenic leukocytes. BALB/c splenic leukocytes were activated with lipopolysaccharide LPS; 72 hrs . The activated cells were then preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 and then stained with either BD Horizon R718 Rat IgG2a, kappa Isotype Control Cat. No. 566941; dashed line histogram or BD Horizon R718 Rat Anti-Mouse CD86 B7-2 antibody Cat. No. 570981/571052; solid line histogram at 0.5 mug/test. DAPI Solution Cat. No. 564907 was added to cells right before analysis. The fluorescence histogram showing CD86 B7-2 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.