BD HorizonTM R718 Mouse Anti-Rat CD90/Mouse CD90.1

Description

Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD90 expression on rat splenocytes . Lewis rat splenic leucocytes were preincubated with Purified Mouse Anti-Rat CD32 antibody Rat BD Fc Block Cat. No. 550270/550271 . The cells were stained with PE Mouse Anti-Rat CD3 antibody Cat. No. 554833 and either BD Horizon R718 Mouse IgG1, kappa Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Rat CD90/Mouse CD90.1 antibody Cat. No. 567361; Right Plot at 0.5 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD90 or Ig Isotype control staining versus CD3 was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.