
566976 ·
BDB Bioscience · Cat: 566976
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Description
Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Granzyme A expression in human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 . The cells were then washed and stained in BD Perm/Wash Buffer Cat. No. 554723 with either BD Horizon R718 Mouse IgG1 Isotype Control Cat. No. 566928; Left Plot or BD Horizon R718 Mouse Anti-Human Granzyme A antibody Cat. No. 566976; Right Plot . A bivariate pseudocolor density plot showing the correlated expression of Granzyme A or Ig Isotype control staining versus side light-scatter SSC signals was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Flow Cytometer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.