BD HorizonTM PE-CF594 Rat Anti-Mouse CD86

Description

Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD86 expression on resting or activated mouse splenocytes. Freshly isolated Left Panel or 72-hour lipopolysaccharide-stimulated Right Panel mouse splenic leucocytes were pretreated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with either BD Horizon PE-CF594 Rat IgG2a, kappa Isotype Control Cat. No. 562302; dashed line histograms or BD Horizon PE-CF594 Rat Anti-Mouse CD86 antibody Cat. No. 567592; solid line histograms at 0.25 mug/test. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable resting Left Panel or activated Right Panel lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.