BD HorizonTM PE-CF594 Rat Anti-Mouse CD39

Description

Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 , APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 , and either BD Horizon PE-CF594 Rat IgG2b, kappa Isotype Control Cat. No. 562308; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD39 antibody Cat. No. 567268; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD25 staining were derived from CD4-positive viable lymphocytes. Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 , APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 , and either BD Horizon PE-CF594 Rat IgG2b, kappa Isotype Control Cat. No. 562308; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD39 antibody Cat. No. 567268; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD25 staining were derived from CD4-positive viable lymphocytes. Show More Multicolor flow cytometric analysis of CD39 expression on Mouse splenic lymphocytes. Balb/c mouse splenocytes were stained with BD Horizon BUV395 Rat Anti-Mouse CD4 Cat. No. 563790/565974 , APC Rat Anti-Mouse CD25 Cat. No. 557192/561048 , and either BD Horizon PE-CF594 Rat IgG2b, kappa Isotype Control Cat. No. 562308; Left Plots or BD Horizon PE-CF594 Rat Anti-Mouse CD39 antibody Cat. No. 567268; Right Plots at 0.5 mug/test. 7-AAD 7-Amino-Actinomycin D Solution Cat. No. 559925 was added to cells right before analysis. Flow cytometry and data analysis were performed using a BD X-20 LSRFortessa Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Upper panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD4 staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative lymphocytes. Lower panels: Bivariate pseudocolor density plots showing correlated expression of CD39 or Ig Isotype control versus CD25 staining were derived from CD4-positive viable lymphocytes.