BD HorizonTM PE-CF594 Mouse Anti-Human LAG-3 (CD223)

Description

Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated and stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated Top Plots or stimulated Bottom Plots PBMC from the same donor were stained with APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935 , and BD Horizon PE-CF594 Mouse Anti-Human LAG-3 CD223 Cat. No. 565718/565719 antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots , or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated Top Plots or stimulated Bottom Plots lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated and stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated Top Plots or stimulated Bottom Plots PBMC from the same donor were stained with APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935 , and BD Horizon PE-CF594 Mouse Anti-Human LAG-3 CD223 Cat. No. 565718/565719 antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots , or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated Top Plots or stimulated Bottom Plots lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Multicolor flow cytometric analysis of LAG-3 CD223 expression on unstimulated and stimulated human peripheral blood lymphocytes. Human peripheral blood mononuclear cells PBMC were cultured for 3 days with plate-bound Anti-Human CD3 Cat. No. 555329; 10 mug/mL for coating and soluble Anti-Human CD28 Cat. No. 555725; 1 mug/mL antibodies, and Human Recombinant IL-2 Cat. No. 554603; 10 ng/mL . Unstimulated Top Plots or stimulated Bottom Plots PBMC from the same donor were stained with APC Mouse Anti-Human CD8 Cat. No. 555369/561421/561952/561953 , BD Horizon BV421 Mouse Anti-Human CD279 PD-1 Cat. No. 562516/565935 , and BD Horizon PE-CF594 Mouse Anti-Human LAG-3 CD223 Cat. No. 565718/565719 antibodies. Two-color flow cytometric contour plots showing the correlated expression of LAG-3 CD223 versus CD8 Left Plots , or LAG-3 CD223 versus CD279 PD-1 Right Plots were derived from gated events with the forward and side light-scatter characteristics of viable unstimulated Top Plots or stimulated Bottom Plots lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.