BD HorizonTM Fixable Viability Stain 620

Description

Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown . Show More Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown . Show More Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 620. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 620 Cat. No. 564996 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the forward and side light-scattering characteristics of Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Flow Cytometry System. Please note that FVS620 is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 620 has been tested on mouse data not shown .