BD HorizonTM Fixable Viability Stain 520

Description

Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown . Show More Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown . Show More Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 520. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panel or 5 muM camptothecin Right Panel for 16 hours and then stained with BD Horizon Fixable Viability Stain 520 Cat. No. 564407 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Histograms were derived from gated events with the light scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Please note that FVS520 is also compatible with BD Phosflow Perm Buffer III Cat. No. 558050 and BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Fixable Viability Stain 520 has been tested on mouse data not shown .