BD HorizonTM Fixable Viability Stain 440UV

Description

Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 440UV. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panels or 5 muM camptothecin Right Panels for 16 hours and then stained Bottom Panels with BD Horizon Fixable Viability Stain 440UV Cat. No. 566332 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Dead cell fluorescence is retained after fixation and permeabilization. The shift in the fluorescence of the live cells is due to a shift in auto-fluorescence post-fixation, as evidenced by the shift in the unstained Top Panels samples. Histograms were derived from gated events with the forward and side light-scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometry System. Please note that FVS440UV is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 440UV. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panels or 5 muM camptothecin Right Panels for 16 hours and then stained Bottom Panels with BD Horizon Fixable Viability Stain 440UV Cat. No. 566332 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Dead cell fluorescence is retained after fixation and permeabilization. The shift in the fluorescence of the live cells is due to a shift in auto-fluorescence post-fixation, as evidenced by the shift in the unstained Top Panels samples. Histograms were derived from gated events with the forward and side light-scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometry System. Please note that FVS440UV is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . Show More Flow cytometric analysis of human Jurkat cells stained with BD Horizon Fixable Viability Stain 440UV. Cells from the human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line were treated with 0.025 % DMSO Left Panels or 5 muM camptothecin Right Panels for 16 hours and then stained Bottom Panels with BD Horizon Fixable Viability Stain 440UV Cat. No. 566332 in serum-free buffer. The cells were then either left unfixed solid line histograms or fixed in BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized in BD Phosflow Perm/Wash Buffer I Cat. No. 557885 dashed line histograms . Dead cell fluorescence is retained after fixation and permeabilization. The shift in the fluorescence of the live cells is due to a shift in auto-fluorescence post-fixation, as evidenced by the shift in the unstained Top Panels samples. Histograms were derived from gated events with the forward and side light-scattering characteristics of intact Jurkat cells. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometry System. Please note that FVS440UV is also compatible with BD Phosflow Perm Buffer III Cat. No.558050 or BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 .