BD HorizonTM BV510 Rat Anti-Mouse CD62L

Description

Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Bone marrow cells from a BALB/c mouse were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse CD62L antibody Cat. No. 563117, solid line histogram or with BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952, dashed line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV510 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric dot plots showing the expression of CD62L or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Bone marrow cells from a BALB/c mouse were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse CD62L antibody Cat. No. 563117, solid line histogram or with BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952, dashed line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV510 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric dot plots showing the expression of CD62L or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Left Panels - Flow cytometric analysis of CD62L on mouse bone marrow cells. Bone marrow cells from a BALB/c mouse were left untreated Left Panel or were cultured 1 hour with Phorbol 12-Myristate 13-Acetate PMA; Middle Left Panel . The cells were then stained with either BD Horizon BV510 Rat Anti-Mouse CD62L antibody Cat. No. 563117, solid line histogram or with BD Horizon BV510 Rat IgG2a, kappa Isotype Control Cat. No. 562952, dashed line histogram . Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Right Panels - Multicolor flow cytometric analysis of CD62L expression on mouse splenocytes. Splenic leucocytes from a BALB/c mouse were stained with APC Hamster Anti-Mouse CD3e Cat. No. 553066/561826 and with either BD Horizon BV510 Rat IgG2a, kappa Isotype Control Middle Right Panel or BD Horizon BV510 Rat Anti-Mouse CD62L antibody Right Panel . Two-color flow cytometric dot plots showing the expression of CD62L or Ig Isotype control staining versus CD3 were derived from gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.