BD HorizonTM BV510 Mouse anti-BrdU

Description

Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System. Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System. Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System. Show More Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System. Show More Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System. Two-color flow cytometric analysis of DNA synthesis by TK-1 cells. BrdU labeling and staining of cells was performed using the procedure and reagents, including BrdU, fixation and permeabilization buffers and 7-AAD Staining Solution from the BD Pharmingen FITC BrdU Flow Kit Cat. No. 559619 . Cells from the TK-1 cell line were either labeled with 50 muM BrdU for 1 hour Left Panel or were not labeled Right Panel . The permeabilized cells were stained with BD Horizon BV510 Mouse Anti-BrdU antibody Cat. No. 563445 followed by staining with the 7-AAD solution. Two-color flow cytometric dot plots showing the correlated expression patterns of 7-AAD total cellular DNA versus incorporated BrdU representing newly-synthesized DNA were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometric analysis was performed using a BD LSR II System.