BD HorizonTM BV480 Mouse Anti-Bcl-6

Description

Analysis of Human Bcl-6 Expression Left Panel - Flow cytometric analysis of Bcl-6 expression in Ramos cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BV480 mIgG1, kappa Isotype Control Cat. No. 565652; dashed line histogram or BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; solid line histogram . A fluorescence histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel - Immunohistofluorescence analysis of Bcl-6 expression by cells within human tonsil. A frozen human tonsil section was stained with BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; pseudocolored cyan . After washing, nuclei were counterstained with DRAQ5 Cat. No. 564902/564903; pseudocolored red . Original magnification, 20X. Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. Mouse mesenteric lymph node cells were stained with BD Horizon BUV395 Rat Anti-Mouse B220 Cat. No. 563793 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , PE Hamster Anti-Mouse Fas/CD95 Cat. No. 561985 , and APC-H7 Rat Anti-Mouse IgD Cat. No. 565348 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BV480 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Analysis of Human Bcl-6 Expression Left Panel - Flow cytometric analysis of Bcl-6 expression in Ramos cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BV480 mIgG1, kappa Isotype Control Cat. No. 565652; dashed line histogram or BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; solid line histogram . A fluorescence histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel - Immunohistofluorescence analysis of Bcl-6 expression by cells within human tonsil. A frozen human tonsil section was stained with BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; pseudocolored cyan . After washing, nuclei were counterstained with DRAQ5 Cat. No. 564902/564903; pseudocolored red . Original magnification, 20X. Show More Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. Mouse mesenteric lymph node cells were stained with BD Horizon BUV395 Rat Anti-Mouse B220 Cat. No. 563793 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , PE Hamster Anti-Mouse Fas/CD95 Cat. No. 561985 , and APC-H7 Rat Anti-Mouse IgD Cat. No. 565348 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BV480 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Analysis of Human Bcl-6 Expression Left Panel - Flow cytometric analysis of Bcl-6 expression in Ramos cells. Cells from the human Ramos Burkitts lymphoma, ATCC CRL-1596 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BV480 mIgG1, kappa Isotype Control Cat. No. 565652; dashed line histogram or BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; solid line histogram . A fluorescence histogram showing Bcl-6 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Right Panel - Immunohistofluorescence analysis of Bcl-6 expression by cells within human tonsil. A frozen human tonsil section was stained with BD Horizon BV480 Mouse Anti-Bcl-6 antibody Cat. No. 567717/567718; pseudocolored cyan . After washing, nuclei were counterstained with DRAQ5 Cat. No. 564902/564903; pseudocolored red . Original magnification, 20X. Multicolor flow cytometric analysis of Bcl-6 expression in Mouse B lymphocytes. Mouse mesenteric lymph node cells were stained with BD Horizon BUV395 Rat Anti-Mouse B220 Cat. No. 563793 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , PE Hamster Anti-Mouse Fas/CD95 Cat. No. 561985 , and APC-H7 Rat Anti-Mouse IgD Cat. No. 565348 antibodies. Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BV480 Mouse Anti-Bcl-6 antibody. The bivariate pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the forward and side light-scatter characteristics of intact lymphocytes Left Plot . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Plot derived from gated cell subsets show intracellular Bcl-6 staining levels for mouse GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.