BD HorizonTM BV480 Goat Anti-Rabbit IgG

Description

Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were stained 1 hr with Purified Rabbit Anti-PCNA antibody Abcam at 2.5 mug/mL. After washing, the cells were counterstained 45 min in blocking buffer with the second step reagent, BD Horizon BV480 Anti- Rabbit IgG Cat. No. 564879; 2.5 mug/mL; pseudo-colored green . Cells were then stained 1 hr in blocking buffer with Alexa Fluor 488 Mouse Anti-beta-Tubulin Cat. No. 558605; pseudo-colored red . BD Pharmingen DAPI Solution Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective. Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were stained 1 hr with Purified Rabbit Anti-PCNA antibody Abcam at 2.5 mug/mL. After washing, the cells were counterstained 45 min in blocking buffer with the second step reagent, BD Horizon BV480 Anti- Rabbit IgG Cat. No. 564879; 2.5 mug/mL; pseudo-colored green . Cells were then stained 1 hr in blocking buffer with Alexa Fluor 488 Mouse Anti-beta-Tubulin Cat. No. 558605; pseudo-colored red . BD Pharmingen DAPI Solution Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective. Show More Immunofluorescence staining of human U-2 OS cells. Cultured cells from the human U-2 OS Osteosarcoma, ATCC HTB-96 cell line were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , permeabilized 5 min with BD Phosflow Perm Buffer III Cat. No. 558050 , washed with 1x PBS, and blocked 30 min with 5 % Goat serum, 1 % BSA, and 0.5 % Triton X-100 diluted in 1x PBS. The cells were stained 1 hr with Purified Rabbit Anti-PCNA antibody Abcam at 2.5 mug/mL. After washing, the cells were counterstained 45 min in blocking buffer with the second step reagent, BD Horizon BV480 Anti- Rabbit IgG Cat. No. 564879; 2.5 mug/mL; pseudo-colored green . Cells were then stained 1 hr in blocking buffer with Alexa Fluor 488 Mouse Anti-beta-Tubulin Cat. No. 558605; pseudo-colored red . BD Pharmingen DAPI Solution Cat. No. 564907 was used as a nuclear counterstain pseudo-colored blue . Slides were mounted with ProLong Gold and the image was captured on a BD Pathway 435 Cell Analyzer epifluorescence microscope and merged using BD Attovision Software. 20X objective.