BD HorizonTM BV421 Rat Anti-Mouse Ly-49A

Description

Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of Ly-49A expression on viable Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553142 . The cells were then stained with APC Rat Anti-Mouse CD49b antibody Cat. No. 560628 and with either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603; Left Plot or BD Horizon BV421 Rat Anti-Mouse Ly-49A antibody Cat. No. 570083/570164; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of Ly-49A or Ig Isotype control staining versus CD49b was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative splenic leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.