BD HorizonTM BV421 Rat Anti-Mouse CD278

Description

Two-color flow cytometric analysis of CD278 ICOS expression on activated mouse lymphocytes. BALB/c splenocytes were either unstimulated Top Panels or activated 3 days; Bottom Panels by plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Both sets of cells were stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603, Left Panels or BD Horizon BV421 Rat Anti-Mouse CD278 antibody Cat. No. 564070; Right Panels . Two-color flow cytometric dot plots show the correlated expression patterns of CD278 or Ig Isotype control staining versus CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of CD278 ICOS expression on activated mouse lymphocytes. BALB/c splenocytes were either unstimulated Top Panels or activated 3 days; Bottom Panels by plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Both sets of cells were stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603, Left Panels or BD Horizon BV421 Rat Anti-Mouse CD278 antibody Cat. No. 564070; Right Panels . Two-color flow cytometric dot plots show the correlated expression patterns of CD278 or Ig Isotype control staining versus CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of CD278 ICOS expression on activated mouse lymphocytes. BALB/c splenocytes were either unstimulated Top Panels or activated 3 days; Bottom Panels by plate-bound Purified NA/LE Hamster Anti-Mouse CD3e antibody Cat. No. 553057 . The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . Both sets of cells were stained with PE Rat Anti-Mouse CD8a antibody Cat. No. 553033/553032/561095 and either BD Horizon BV421 Rat IgG2b, kappa Isotype Control Cat. No. 562603, Left Panels or BD Horizon BV421 Rat Anti-Mouse CD278 antibody Cat. No. 564070; Right Panels . Two-color flow cytometric dot plots show the correlated expression patterns of CD278 or Ig Isotype control staining versus CD8a for gated events with the forward and side light-scatter characteristics of viable splenic leucocytes. Flow cytometric analysis was performed using a BD LSR II Flow Cytometer System.