BD HorizonTM BV421 Rat Anti-Mouse CD140b (PDGFRbeta)

Description

Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b PDGFRbeta on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 and PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 Left Plot or with BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126 and PE Rat Anti-Mouse CD140a antibody Cat. No. 562776 Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b PDGFRbeta versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD140b PDGFRbeta expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126; solid line histograms at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD140b PDGFRbeta expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b PDGFRbeta on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 and PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 Left Plot or with BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126 and PE Rat Anti-Mouse CD140a antibody Cat. No. 562776 Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b PDGFRbeta versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of CD140b PDGFRbeta expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126; solid line histograms at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD140b PDGFRbeta expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of the correlated expression of CD140a and CD140b PDGFRbeta on Mouse NIH/3T3 cells. Cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line were stained with BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602 and PE Rat IgG2a, kappa Isotype Control Cat. No. 553930 Left Plot or with BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126 and PE Rat Anti-Mouse CD140a antibody Cat. No. 562776 Right Plot at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD140b PDGFRbeta versus CD140a or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of CD140b PDGFRbeta expression on Mouse splenic leukocytes and Mouse NIH/3T3 cells. C57BL/6 Mouse splenocytes Left Plot were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The splenic leukocytes and cells from the Mouse NIH/3T3 Mouse embryonic fibroblast, ATCC CRL-1658 cell line Right Plot were then stained with either BD Horizon BV421 Rat IgG2a, kappa Isotype Control Cat. No. 562602; dashed line histograms or BD Horizon BV421 Rat Anti-Mouse CD140b PDGFRbeta antibody Cat. No. 570045/570126; solid line histograms at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histograms showing CD140b PDGFRbeta expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.