BD HorizonTM BV421 Mouse Anti-Mouse RORgammat

Description

Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Show More Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System. Multicolor flow cytometric analysis of RORgammat expression in mouse thymocytes. BALB/c mouse thymocytes were stained with APC Rat Anti-Mouse CD4 Cat. No. 553051/561091 and FITC Rat Anti-Mouse CD8a Cat. No. 553031/553030/561966 antibodies, and then fixed and permeabilized using the BD Pharmingen Transcription Factor Buffer Set Cat. No. 562574/562725 . The fixed and permeabilized cells were then stained with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439 or BD Horizon BV421 Mouse Anti-Mouse RORgammat antibody Cat. No. 562894 . The overlapping histograms show the levels of IgG2a Isotype Control Left Panel and RORgammat Right Panel staining in CD4+ CD8- single-positive dashed line histogram or CD4+ CD8+ double-positive solid line histogram thymocytes. The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of intact cells. Flow cytometry was performed using a BD LSR II Flow Cytometry System.