BD HorizonTM BV421 Mouse Anti-Human TNF

Description

Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Multiparameter flow cytometric analysis of TNF expressed in stimulated human peripheral blood mononuclear cells. HiCK-1 Human Cytokine Positive Control Cells Cat. No. 555061 were permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human TNF antibody Cat. No. 562783/566275; Middle Panel . To demonstrate specificity of staining, the fixed and permeabilized cells were preincubated with Purified Mouse Anti-Human TNF antibody 10 mug, Cat. No. 554510; Right Panel to block subsequent staining with the BV421 Mouse Anti-Human TNF antibody. Two-color flow cytometric dot plots show the correlated expression patterns of TNF, Ig Isotype control or blocked TNF staining versus autofluorescence for gated events with the forward and side light-scatter characteristics of intact peripheral blood mononuclear cells. Flow cytometry was performed using a BD LSR II Flow Cytometer System.