BD HorizonTM BV421 Mouse Anti-Human Neutrophil Elastase

Description

Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of Human Neutrophil Elastase expression in Human peripheral blood granulocytes and tumor cell lines. Human peripheral blood granulocytes Left Plot prepared by density gradient centrifugation and cells from the Human Jurkat Acute T cell leukemia, ATCC TIB-152 cell line Middle Plot and Human U-937 Histiocytic lymphoma, ATCC CRL-1593 cell line Right Plot were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 , washed and then permeabilized with BD Perm/Wash Buffer Cat. No. 554723 . The cells were then stained in BD Perm/Wash Buffer with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; dashed line histograms or BD Horizon BV421 Mouse Anti-Human Neutrophil Elastase antibody Cat. No. 569513/569514; solid line histograms at 1 mug/test. The fluorescence histograms showing Neutrophil Elastase expression or Ig Isotype control staining were derived from gated events with the forward and side light-scatter characteristics of intact cells. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.