
562893 ·
BDB Bioscience · Cat: 562893
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Description
Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Show More Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System. Two-color flow cytometric analysis of Immunoglobulin Light Chain lambda expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were incubated in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with FITC Mouse Anti-Human CD19 antibody Cat. No. 555412/560994 and with either BD Horizon BV421 Mouse IgG1, kappa Isotype Control Cat. No. 562438; Left Panel or BD Horizon BV421 Mouse Anti-Human Light Chain, lambda antibody Cat. No. 562893; Right Panel . Two-color flow cytometric dot plots show the correlated expression of Human Immunoglobulin Light Chain lambda or Ig Isotype control staining versus CD19 for gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD LSR II Flow Cytometer System.