BD HorizonTM BV421 Mouse Anti-Human GITR (CD357)

Description

Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium alone top left panel or in medium with PHA-M form 1.5 % v/v; top right panel . The cells were then stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; not shown or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; both panels . The two-parameter flow cytometric contour plots showing the correlated expression of CD4 and GITR CD357 were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes. No staining was observed with the Ig Isotype control. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4-positive peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , APC Mouse Anti-Human CD25 Cat. No. 555434 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histograms or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; solid line histograms . Events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes and expression of CD4 were gated. These cells were further gated based on the absence bottom left panel or presence bottom right panel of CD25 expression. The overlayed histograms depict GITR CD357 expression solid line versus Ig Isotype control staining dashed line . Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium alone top left panel or in medium with PHA-M form 1.5 % v/v; top right panel . The cells were then stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; not shown or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; both panels . The two-parameter flow cytometric contour plots showing the correlated expression of CD4 and GITR CD357 were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes. No staining was observed with the Ig Isotype control. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4-positive peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , APC Mouse Anti-Human CD25 Cat. No. 555434 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histograms or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; solid line histograms . Events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes and expression of CD4 were gated. These cells were further gated based on the absence bottom left panel or presence bottom right panel of CD25 expression. The overlayed histograms depict GITR CD357 expression solid line versus Ig Isotype control staining dashed line . Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Show More Multiparameter flow cytometric analysis of GITR CD357 expression on human peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were cultured for 3 days in medium alone top left panel or in medium with PHA-M form 1.5 % v/v; top right panel . The cells were then stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; not shown or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; both panels . The two-parameter flow cytometric contour plots showing the correlated expression of CD4 and GITR CD357 were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes. No staining was observed with the Ig Isotype control. Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System. Multiparameter flow cytometric analysis of GITR CD357 expression on human CD4-positive peripheral blood mononuclear cells. Human peripheral blood mononuclear cells were stained with BD Horizon BUV395 Mouse Anti-Human CD4 Cat. No. 564724 , APC Mouse Anti-Human CD25 Cat. No. 555434 and with either BD Horizon BV421 Mouse IgG2b, kappa Isotype Control Cat. No. 562748; dashed line histograms or BD Horizon BV421 Mouse Anti-Human GITR CD357 Cat. No. 566423; solid line histograms . Events with the forward and side light-scatter characteristics of viable human peripheral blood mononuclear leucocytes and expression of CD4 were gated. These cells were further gated based on the absence bottom left panel or presence bottom right panel of CD25 expression. The overlayed histograms depict GITR CD357 expression solid line versus Ig Isotype control staining dashed line . Flow cytometric analysis was performed using a BD LSRFortessa X-20 Flow Cytometer System.