
568323 · 100 Tests
BDB Bioscience · Cat: 568323
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bdbiosciences.com/en-us/products/reagents/flow-cytometry-rea...Description
Multiparameter flow cytometric analysis of CD7 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD3 antibody Cat. No. 566953 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plots or BD Horizon BV421 Mouse Anti-Human CD7 antibody Cat. No. 568323/568324; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either CD7 or Ig Isotype control staining versus CD3 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Multiparameter flow cytometric analysis of CD7 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD3 antibody Cat. No. 566953 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plots or BD Horizon BV421 Mouse Anti-Human CD7 antibody Cat. No. 568323/568324; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either CD7 or Ig Isotype control staining versus CD3 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Show More Multiparameter flow cytometric analysis of CD7 expression on Human peripheral blood leucocytes. Human whole blood was stained with BD Horizon R718 Mouse Anti-Human CD3 antibody Cat. No. 566953 and with either BD Horizon BV421 Mouse IgG2a, kappa Isotype Control Cat. No. 562439; Left Plots or BD Horizon BV421 Mouse Anti-Human CD7 antibody Cat. No. 568323/568324; Right Plots . Erythrocytes were lysed with BD FACS Lysing Solution Cat. No. 349202 . The bivariate pseudocolor density plots showing the correlated expression of either CD7 or Ig Isotype control staining versus CD3 Top Plots or side light-scatter SSC-A signals Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes Top Plots or leucocyte populations Bottom Plots . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software.