BD HorizonTM BUV737 Rat Anti-Mouse I-A/I-E

Description

Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of I-A/I-E MHC Class II alloantigen expression on viable Mouse splenic leucocytes. C57BL/6 Mouse splenocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with PerCP-Cy5.5 Hamster Anti-Mouse CD3e antibody Cat. No. 551163/561108 and with either BD Horizon BUV737 Rat IgG2b, kappa Isotype Control Cat. No. 612762; Left Plot or BD Horizon BUV737 Rat Anti-Mouse I-A/I-E antibody Cat. No. 569176; Right Plot at 0.25 mug/test. DAPI 4,6-Diamidino-2-Phenylindole, Dihydrochloride Solution Cat. No. 564907 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of I-A/I-E or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable DAPI-negative leucocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.