BD HorizonTM BUV737 Hamster Anti-Mouse CD103 (Integrin alphaE)

Description

Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Show More Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific. Multicolor flow cytometric analysis of CD103 Integrin alphaE expression on Mouse lymph node cells. C57BL/6 Mouse lymph node cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/553142 . The cells were then stained with BD Horizon PE-CF594 Hamster Anti-Mouse CD3e antibody Cat. No. 562286/562332 and with either BD Horizon BUV737 Hamster IgG2, kappa Isotype Control Cat. No. 612774; Left Plot or BD Horizon BUV737 Hamster Anti-Mouse CD103 Integrin alphaE antibody Cat. No. 569678; Right Plot at 1 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD103 Integrin alphaE or Ig Isotype control staining versus CD3e was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative leukocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo Software. Data shown on this Technical Data Sheet are not lot specific.