BD HorizonTM BUV563 Mouse Anti-Human ICOS (CD278)

Description

Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV563 Mouse IgG1 kappa Isotype Control Cat. No. 612920; dashed line histogram or BD Horizon BUV563 Mouse Anti-Human ICOS CD278 antibody Cat. No. 568215; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV563 Mouse IgG1 kappa Isotype Control Cat. No. 612920; dashed line histogram or BD Horizon BUV563 Mouse Anti-Human ICOS CD278 antibody Cat. No. 568215; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific. Show More Flow cytometric analysis of ICOS CD278 expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin PHA -stimulated 3 days peripheral blood mononuclear cells were stained with either BD Horizon BUV563 Mouse IgG1 kappa Isotype Control Cat. No. 612920; dashed line histogram or BD Horizon BUV563 Mouse Anti-Human ICOS CD278 antibody Cat. No. 568215; solid line histogram at 0.25 mug/test. BD Via-Probe Cell Viability 7-AAD Solution Cat. No. 555815/555816 was added to cells right before analysis. The fluorescence histogram showing ICOS CD278 expression or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of viable 7-AAD-negative stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 Cell Analyzer System and FlowJo software. Data shown on this Technical Data Sheet are not lot specific.