BD HorizonTM BUV496 Mouse Anti-Bcl-6

Description

Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software. Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software. Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software. Show More Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software. Show More Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software. Bcl-6 expression in Ramos cells Left Panel . Human Ramos Burkitts lymphoma, ATCC CRL-1596 cells were fixed with BD Cytofix Fixation Buffer Cat. No. 554655 and permeabilized with BD Phosflow Perm Buffer III Cat. No. 558050 followed by intracellular staining with either BD Horizon BUV496 Mouse IgG1, kappa Isotype Control Cat. No. 612949; dashed line histogram or BD Horizon BUV496 Mouse Anti-Human Bcl-6 antibody Cat. No. 567752/567753, solid line histogram . The fluorescence histogram showing the expression of Bcl-6 or Ig Isotype control staining was derived from gated events with the forward and side light-scatter characteristics of intact cells. Bcl-6 expression in mouse B cells Middle and Right Panels . Mouse mesenteric lymph node cells were stained with PE Rat Anti-Mouse B220 Cat. No. 553090 , Alexa Fluor 700 Rat Anti-Mouse CD4 Cat. No. 557956 , BD Horizon BV786 Hamster Anti-Mouse Fas/CD95 Cat. No. 740906 , and BD Horizon BUV395 Rat Anti-Mouse IgD Cat. No. 565988 . Cells were washed, resuspended in RPMI 1640 medium, and fixed with BD Phosflow Lyse/Fix Buffer Cat. No. 558049 . Cells were permeabilized with BD Phosflow Perm/Wash Buffer I Cat. No. 557885 , followed by intracellular staining with BD Horizon BUV496 Mouse Anti-Bcl-6 antibody. The pseudocolor density plot showing the correlated expression of IgD versus Fas/CD95 on B cells was derived from CD4-B220+ gated events with the light-scatter characteristics of intact lymphocytes Middle Panel . Germinal center GC B cells were identified as Fas/CD95-positive B cells that expressed low levels of IgD IgDloCD95/Fas+ whereas non-GC B cells primarily expressed intermediate to high levels of IgD and little or no CD95/Fas. Histograms Right Panel derived from gated cell subsets show intracellular Bcl-6 staining levels for GC B cells solid line and non-GC B cells dashed line . Flow cytometry and data analysis were performed using a BD LSRFortessa X-20 and FlowJo software.