BD HorizonTM BUV395 Mouse Anti-Human IL-3Ralpha (CD123)

Description

Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals Human Leucocytes; Top Plots or CD45RO Human Lymphocytes; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes or lymphocytes. Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Top Plots: Human Leucocytes. Human whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567277/567278; Right Plot . Bottom Plots: Human Lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899; Bottom Plots and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Right Plot . Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals Human Leucocytes; Top Plots or CD45RO Human Lymphocytes; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes or lymphocytes. Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Top Plots: Human Leucocytes. Human whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567277/567278; Right Plot . Bottom Plots: Human Lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899; Bottom Plots and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Right Plot . Show More Multiparameter flow cytometric analysis of IL-3Ralpha CD123 expression on human peripheral blood leucocyte populations. Bivariate pseudocolor density plots showing the correlated expression of IL-3Ralpha CD123 or Ig Isotype control staining versus side light-scatter SSC-A signals Human Leucocytes; Top Plots or CD45RO Human Lymphocytes; Bottom Plots were derived from gated events with the forward and side light-scatter characteristics of intact human leucocytes or lymphocytes. Erythrocytes were lysed with BD FACS Lysing Solution Cat No. 349202 . Flow cytometry and data analysis were performed using a BD LSRFortessa Cell Analyzer System and FlowJo software. Top Plots: Human Leucocytes. Human whole blood was stained with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Cat No. 563547; Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Cat No. 567277/567278; Right Plot . Bottom Plots: Human Lymphocytes. Human whole blood was stained with APC Mouse Anti-Human CD45RO Cat No. 559865/560899; Bottom Plots and with either BD Horizon BUV395 Mouse IgG1, kappa Isotype Control Left Plot or BD Horizon BUV395 Mouse Anti-Human IL-3Ralpha CD123 antibody Right Plot .