BD HorizonTM APC-R700 Rat Anti-Mouse CD25

Description

Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Show More Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System. Flow cytometric analysis of CD25 expression on unstimulated and stimulated mouse splenocytes. Left and Middle Panels: Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody Mouse BD Fc Block Cat. No. 553141/5 53142 . The cells were then stained with FITC Rat Anti-Mouse CD4 antibody Cat. No. 553047/553046/561835 and either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control Cat. No. 565133; Left Panel or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody Cat. No. 565134/565135; Middle Panel . Two-color flow cytometric dot plots showing the correlated expression of CD25 or Ig Isotype control staining versus CD4 were derived from gated events with the forward and side light-scatter characteristics of viable splenocytes. Right Panel: Mouse splenic leucocytes were stimulated for 3 days with concanavalin A. The cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody and then stained with either BD Horizon APC-R700 Rat IgG1, lambda Isotype Control dashed line histogram or BD Horizon APC-R700 Rat Anti-Mouse CD25 antibody solid line histogram . The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable lymphoblasts. Flow cytometric analysis was performed using a BD LSRFortessa Cell Analyzer System.