ANTIBIOTIC ASSAY MEDIUM NO. 8 (BASE AGAR W/ LOW pH) (VEG.)

Description

Antibiotic Veg Assay Medium No. 8 is prepared incorporating vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. This medium can be used for the same purpose of Antibiotic Assay Medium No. 8, for the assay of various antibiotics. Grove and Randall have elaborately elucidated the methods to perform these assays and various media used for the same. Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay. These media are also recommended by USP and FDA. Similarly, Antibiotic Veg Assay Medium No. 8, used especially to prepare the base layer to assay Tetracyclines and other antibiotics. It provides a solidified substratum for growth of organisms. The medium has an optimal pH of 5.9 for assay of Tetracycline as these antibiotics are stable at slightly lower pH. This pH condition also supports the growth of test organisms. Antibiotic Veg Assay Medium No. 8 is also used as base and seed agar medium for agar diffusion assay for Mitomycin, Mithramycin, Plicamycin and Vancomyci