ANTIBIOTIC ASSAY MEDIUM NO. 5 (STREPTOMYCIN ASSAY AGAR) (VEG.)

Description

Antibiotic Veg Assay Medium No. 5 (Streptomycin Veg Assay Agar w/Yeast Extract) is prepared by incorporating vegetable peptones in place of animal peptones, making the medium BSE, TSE risks free. It can be used for the same purpose of Antibiotic Assay Medium No.5. Groove and Randall had elucidated the methods to perform these assays. This medium is recommended for assaying Streptomycin by cylinder plate using Bacillus subtilis as test organism. It can be used in the assay of commercial preparations of antibiotics as well as for antibiotics in body fluids, feeds etc. This medium can also be used to prepare the base as well as seed layer in the microbiological assay of antibiotics such as Dihydrostreptomycin, Framycetin, Dactinomycin, Streptomycin and Kanamycin B. The pH of 7.9 provides optimum conditions for Bacillus subtilis. To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized test culture can be overlaid. Even distribution of the layer is critical.