ANTIBIOTIC ASSAY MEDIUM NO. 12 (NYSTATIN ASSAY AGAR) (VEG.)

Description

Antibiotic Veg Assay Medium No.12 (Nystatin Veg Assay Agar) is prepared incorporating vegetable peptones in place of animal peptones, making the medium BSE-TSE risks free. It can be used for the same purpose of Antibiotic Assay Medium No.12 (Nystatin Assay Agar). This medium is prepared from the Groove and Randall formula. Antifungal antibiotics like Amphotericin B and Nystatin can be assayed using this medium. Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterilised agar pre-cooled to 40-45?C and spread evenly over the surface of solidified base agar. Prediffusion of antibiotics for 10-20 minutes in the agar by incubating at temperature below the optimal growth temperature for microorganism would facilitate better diffusion of antibiotics followed by incubation of plates for microbial growth.