Anti-DYKDDDDK tag Affinity Beads (Binds to FLAG tag sequence)

Description

Anti-DYKDDDDK tag Affinity Beads Binds to FLAG tag sequence ab270704 is an affinity chromatography medium designed for easy, one-step purification or immunoprecipitation of DYKDDDDK flag at N-terminal, C-terminal and internal locations of a fusion protein. Anti-DYKDDDDK Affinity Beads are coupled with an anti-DYKDDDDK monoclonal antibody by a technique which generates a stable linkage between the antibody and the agarose beads. Characteristics of the resin: Item Description Matrix 4 % agarose beads Ligand Anti-DYKDDDDK Antibody Binding Capacity >1mg DYKDDDDK fusion protein/mL medium Particle size mum 45165 Max Pressure 0.1 MPa, 1 bar Storage Buffer PBS buffer with 0.02 % sodium azide Storage Temperature 4C For the purification of DYKDDDDK-tagged fusion proteins, Anti-DYKDDDDK tag Affinity Beads can be used in column chromatography, batch format and immunoprecipitation. For large volumes of sample, column chromatography or batch format are recommended to quickly capture the target protein from a large volume of extract. When a small amount of sample is being purified, immunoprecipitation procedure is recommended. This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Anti-DYKDDDDK Affinity Beads. Expedeon product codes AF0001 1 mL Medium , AF0005 5 mL Medium , AF0010 10mL Medium , AF0025 25 mL Medium , and AF0050 50 mL Medium are the same as the current sizes: 1 mL, 5 mL, 10 mL, 25 mL, and 50 mL, respectively.