All-In-One 5X RT MasterMix with gDNA Removal

Description

The All-In-One 5X RT MasterMix with gDNA Removal offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, all in a single tube. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis. The optimized MasterMix includes abms proprietary OneScript Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo dT s and Random Primers. The OneScript Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for enhanced cDNA yield and integrity. By eliminating the need for multiple separate reagents, this all-in-one mix offers a straightforward, reliable, and reproducible workflow that consistently delivers high-quality cDNA, even from challenging or low-quality RNA samplesideal for a wide range of downstream molecular biology applications. Product Features: Easy one-step setup reduces pipetting and sample handling Removes contaminating gDNA with temperature-sensitive DNase High cDNA yields from difficult samples with OneScript Hot Reverse Transcriptase Cat. No. G593 Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor Cat. No. G138 Also includes dNTPs, Oligo dT s, and random primers Included in abms PCR Buffet Program Product Component Quantity All-In-One 5X RT MasterMix 100 rxn 400 mul Nuclease-Free H2O 1.0 ml ISO 13485:2016 MDSAP Certified Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek a MDSAP recognized auditing organization .