AAVpro Extraction Solution

Description

Isolation of AAV particles from AAV particle-producing cells is conventionally performed using the freeze-thaw or sonication methods. However, these methods are time consuming and require special equipment. The AAVpro Extraction Solution is a reagent for the extraction of AAV particles from AAV particle-producing cells. This reagent provides a simple and efficient method for AAV particle isolation; the reagents are added to virus-producing cells and viral particles are recovered by centrifugation. The extracted AAV particle solution contains only a small amount of host protein and nucleic acid contamination. The resulting viral extract is well-suited for cell infection or further purification of viral particles. Our products are to be used forResearch Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval. Back Comparison of AAV Extraction Solution with the freeze-thaw method.The titer of AAV2 extract solutions was determined by real-time PCR vector genome; vg . Then, the equivalent of 1x109vg of the AAV2 extract solution was analyzed by SDS-PAGE to evaluate protein impurity Panel A . In addition, residual dsDNA content was assayed using the intercalation method Panel B . The use of the AAV Extraction Solution clearly reduced the amount of protein impurities and dsDNA in comparison with the freeze-thaw method. Back Extraction of serotype 1, 2, and 6 AAV particles.AAV particles were extracted from HEK 293 cells using either the AAVpro Extraction Solution or the freeze-thaw method. The AAVpro Titration Kit for Real Time PCR Ver. 2 Cat. # 6233 was used to measure the titer of the AAV solutions. The results indicate that the AAVpro Extraction Solution can be used to efficiently extract AAV particles that are serotype 1, 2, or 6. Back