LIF (mBA): sc-4989

Description

mature leukemia inhibitory factor LIF of mouse origin, lyophilized available as 10 6 units 10 Amug sc-4989 or 5x10 6 units 50 Amug sc-4989A potent inhibitor of spontaneous embryonic mouse stem cell differentiation and useful for in vitro maintenance of the pluripotential phenotype of ES cells 1000 U per 1 ml of tissue culture media is required to maintain mouse ES cells with a stem cell phenotype; 10 6 units is sufficient for 1 L of tissue culture media, 10 7 units is sufficient for 10 L of tissue culture media produced in E. coli as a GST-tagged fusion protein; GST tag is cleaved and LIF is purified by HPLC chromatography >95 % pure by SDS-PAGE and 0.22 micron sterile filtered prior to lyophilization; tested negative for mycoplasmas Mature, biologically active, recombinant LIF is indistinguishable from native LIF in its biological activities in vitro. Leukemia Inhibitory Factor LIF is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinergic neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo. See product citations 4