A-431 + PMA nuclear extract: sc-2123

Description

supplied in four vials, each containing 250 Amug nuclear extract in 50 Amul buffer provided in 20 mM HEPES pH 7.9 , 20 % v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT human nuclear extract; epidermoid carcinoma cells induced with phorbol suitable for use in Gel Shift and Western Blotting assays Extracts should be stored at -70AC and repeated freezing and thawing should be avoided. prepared by the method of Dignam et al., 1983 Nucleic Acids Res. 11: 1475